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Advanced science: mitochondrial targeted aggregation induced luminescence probe for nondestructive identification of circulating tumor cells

wallpapers News 2020-07-13

circulating tumor cells (CTCs) are tumor cells that shed from the primary tumor enter the peripheral blood. They can spread to all parts of the body through blood circulation form metastases. In addition CTCs also carry a lot of information of tumor tissue in situ. By obtaining CTCs in blood we can monitor the occurrence development of tumor in situ in a non-invasive way which has important research significance clinical application value in tumor management. However there are many kinds of cells in the blood. How to accurately identify the real tumor cells has always been an important problem for researchers. For a long time the identification of CTCS has been limited to the "gold stard" - the detection of cytokeratins (CKs) or fluorescence in situ hybridization (FISH) based on chromosomal abnormalities of tumor cells. Unfortunately the above two methods require cell fixation penetration other chemical staining treatment which greatly damages the nucleic acid composition of cells. Many mutations or genetic information of tumor tissues in situ can not be detected interpreted which can only bring simple cell count data which is difficult to meet the personalized treatment needs of precision medicine. The establishment of

identification methods is back to the "everyone to find fault" between tumor cells white blood cells. Mitochondria are important organelles involved in energy metabolism. Because of its malignant growth characteristics tumor cells have more vigorous life activities than ordinary blood cells which have been proved to have more mitochondria higher mitochondrial membrane potential. Using a new fluorescent probe from Southern University of science technology Tang Zhong Lei his team from Southern University of science technology have successfully used it. TPN is an organic small molecular probe with mitochondrial targeting function which can quickly enter mitochondria of living cells emit bright yellow fluorescence. Compared with mitotracker green TPN labeled cells have higher fluorescence intensity signal-to-noise ratio. Researchers have confirmed in a variety of different tumor cell lines that the fluorescence difference between TPN labeled tumor cells white blood cells can reach 3-12 times making it easy to identify tumor cells mixed with white blood cells. Single cell analysis confirmed that TPN labeled single cells whether DNA or RNA had higher amplification efficiency than traditional labeling methods. The research team further combined with the expression karyotype of leukocyte specific antigen CD45 to establish a new method for the identification of CTCS. This method successfully detected tumor cells from pleural effusion of lung cancer patients further detected 53 cases (77.9%) 11 cases (50%) of CTCS in the blood of 68 lung cancer patients 22 liver cancer patients respectively which further confirmed the application prospect of this method.

this study provides a powerful tool for the non-destructive identification of CTCS high-quality single cell downstream analysis. The researchers believe that the functional difference of tumor cells will be an important direction in the detection research of CTCS.

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